Metallo-carbonyl Probes for Infrared Spectroscopy

We have synthesized and/or studied the reactivity of three series of lysine- and cysteine-targeted metallo-carbonyl complexes with several proteins including BSA, a monoclonal anti-TSH antibody and avidin. Their properties and the IR characteristics of the labeled proteins are gathered in Table 6.2. 

The first series of metallo-carbonyl protein labeling reagents was designed from the popular Bolton-Hunter radio-iodination reagent 48 intended for antibody radiolabeling [85]. 

Metallo-carbonyl N-hydroxysuccinimide esters 49 [86], 50 [87] and 51-53 [88] were synthesized and characterized by classical spectroscopic methods (Fig. 6.9). 

63 Cysteines (neutral pH, r.t.) Cysteines and histidines (neutral pH, 35°C) Cysteines, histidines and lysines (basic pH, 35°C) 2050, 2000 [102] (synthesis) [87] (labelling of BSA) [90,115] (labelling of dendrimer PAMAM G4) 

64 Lysines and/or hishdines 2058,1993 [104] (labelling of chymotrypsin, RNase, lipase, alkaline phosphatase) 

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