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Metabolism ligation reactions

To date, four bioorthogonal reactions have been used to label glycans on cells and in lysates hydrazone/oxime formation with ketones, thiol alkylation with maleimides, Staudinger ligation of azides with triaryl phosphines, and copper-catalyzed or strain-promoted [3+2] cycloadditions of alkynes and azides (Figure 5) (25, 26, 32, 34, 35). While each reaction has been used extensively, most recent applications have employed azido- or alkynyl-sugars due to their superior metabolic incorporation and efficient ligations. [Pg.260]

In Table 14.3, six types of chemical reactions are listed. In metabolic pathways, these reactions would be catalyzed by enzymes. Enzymes are given functional names. For example, isomerization is catalyzed by an isomerase. What would be the enzyme name, ending in -ase for the following oxidation, ligation, group transfer, hydrolytic. [Pg.234]


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See also in sourсe #XX -- [ Pg.425 ]




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Metabolic reactions

Metabolism reactions

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