Mercaptoethanol as reagent

Kiss and Sass-Kiss (2005) developed an on-line post-column derivatization method using OPA and 2-mercaptoethanol as reagents. HPLC... 

Mercaptoethanesnlfonic acid as reagent 23, 36 suppl. 27 Mercaptoethanol as reagent 27, 259... 

The most widely appHed colorimetric assay for amino acids rehes upon ninhydrin-mediated color formation (129). Fluorescamine [38183-12-9] and (9-phthalaldehyde [643-79-8] are popular as fluorescence reagents. The latter reagent, ia conjunction with 2-mercaptoethanol, is most often used ia post-column detection of amino acids separated by conventional automated amino acid analysis. More recently, determiaation by capillary 2one electrophoresis has been developed and it is possible to determine attomole quantities of amino acids (130). 

The Npys group can be. cleaved reductively with BU3P, H2O or mercaptoethanol. It is stable to CF3COOH (24 h), 4 M HCl/dioxane (24 h), and HF (1 h). The related reagent, 2-pyridinesulfenyl chloride, has also been proposed as a useful reagent for the deprotection of the 5-trityl, 5-diphenylmethyl, 5-acetamidomethyl, 5-/-butyl, and S-r-butylsulfenyl groups, but this reagent is very susceptible to hydrolysis. ... 

Note o-Phthaldehyde in the presence of mercaptoethanol or cysteine has already been discussed as a reagent [4]. The present monograph describes the use of o-phthal-aldehyde in the presence of sulfuric add. There are, in addition, a number of applications, which have been described, employing o-phthalaldehyde without any additives e. g. for the detection of primary arylamines, histamine, histidine and histidylpeptides [5-71. 

Add 3 ml of the detergent Brij-35 (as a 30 percent solution) and 2 ml of 2-mercaptoethanol to 950 ml of Fluoraldehyde Reagent Diluent (all reagents from Thermo Fisher). 

After a carrier protein has been activated with sulfo-SMCC, it is often useful to measure the degree of maleimide incorporation prior to coupling an expensive hapten. Ellman s reagent may be used in an indirect method to assess the level of maleimide activity of sulfo-SMCC-activated proteins and other carriers. First, a sulfhydryl-containing compound such as 2-mercaptoethanol or cysteine is reacted in excess with the activated protein. The amount of unreacted sulfhydryls remaining in solution is then determined using the Ellman s reaction (Chapter 1, Section 4.1). Comparison of the response of the sample to a blank reaction using... 

The cells are lysed in a buffer containing strong chaotropic reagents such as guanidine thiocyanate and 2-mercaptoethanol, which completely denatures any ribonuclease present. The supernatant is then placed on a cushion of CsCl (5.7 mol l-1) and centrifuged at 100000 g for 18 h. The RNA passes through the CsCl and is pelleted, while the DNA and protein remain in the aqueous solution. The RNA pellet is dissolved in buffer and concentrated by precipitation in cold ethanol. 

Each tube will have the following components single protein or protein mixture, SDS, 2-mercaptoethanol, and buffer. Add all reagents as indicated and cap each tube tightly. Mix well with gentle shaking and place in a boiling-water bath for 3 minutes. Cool to room temperature and load 10 jA. of a sample onto the top of the appropriate sample well (lane) as follows ... 

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