Membrane lipids mung bean

Fig. 3A. The change in t, of the spin labels of 12-nitroxide methyl stearate (I2NS) (open circles) and 16-nitroxide methyl stearate (I6NS) (solid circles) with mitochondrial membranes of mung bean (V. radiata) as a fimction of temperature. The spin labels were mixed with a suspension of the membranes at a molar ratio of about I spin label per ISO lipids. The motion of the spin label was calculated from first-derivative esr spectra determined over a temperature range of 16°-40°C for 12NS and 0°-38°C 16NS. The straight lines were computed by linear regression analysis, and the temperature for the change in slope of the straight lines determined by a statistical procedure described by Raison and Chapman (1976). Data bom Raison and Chapman (1976).

Versteeg (8) speculated on the function of PE in vivo. He noted the high activity of PE in citrus fruit compared to the amount of available pectin. The fruit contain sufficient activity to deesterify the pectin to low methoxy pectin in 10 min at optimum pH. He suggested that the methyl transferase found by Kauss and Hassid (39) to esterify pectic acid to pectin in mung bean shoots and to be located in a lipid-membrane complex (31) functioned as pectinesterase after the lipid membranes were destroyed and the environment changed. However, no definitive experiments to establish the role of PE in fruits were reported. 

Yoshida, S., Washio, K., Kenrick, J., and Orr, G. (1988) Thermotropic properties of lipids extracted from plasma membrane and tonoplast isolated from chilling-sensitive mung bean (Vigna radiata L.] Wilczek). Plant Cell Physiol. 29,1411-1416. 

Yoshida, S. and Uemura, M. Lipid composition of plasma membrane and tonoplasts isolated from etiolated seedlings of mung bean (Vigna radiata L.), Plant Physiol. 82... 

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