Membrane, artificial lateral diffusion

Vaz WLC, Derzko ZI, Jacobson K. Photobleaching measurements of the lateral diffusion of lipids and proteins in artificial phospholipid bilayer membranes. Cell Surface Rev. 1982 8 83-135. 

SPT permits to study the influence of lateral diffusion in the organization of a large number of membrane proteins. The technique has been limited until lately to observations in cell culture. Only a recent paper showed SPT in brain slices, using spinning disk microscopy to obtain the required high temporal and spatial resolution [25]. Still it has to be kept in mind that the antibody-QD complex has the size of tens of nanometers resulting in steric hindrance and thereby in artificial diffusion barriers, limiting the potential use of conventional QDs in brain tissue. 

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