Mechanical aeration filtration

Intermediary metabolism of irradiated tissues was examined to attempt to explain the increased respiration on the basis of biochemical mechanics. In these experiments carrot disks 0.5 mm. thick were prepared as before and subjected to substrate utilization studies (9). This involved incubating the slices with the appropriately labeled substrate in 0.03M phospate buffer at 25°C. The incubation involves shaking with gentle aeration and trapping the respired CO2 in alkali. Following incubation the slices were removed, washed, ground with 80% ethanol, and filtered, and both the filtrate and residue were assayed for radioactivity. Thus, a measure of substrate absorption and utilization was determined. 

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