Measurement of Peptide Bond Hydrolysis

Several physical and chemical methods have been used to follow the course of hydrolysis of proteins. Each method depends upon measurement of the disappearance of protein or the appearance of hydrolytic products during the course of the reaction. The choice of a method depends upon specific factors which are unique to each experimental situation, and one must choose a method in light of the information desired. 

Several methods can be employed that measure the amount of protein which remains after hydrolysis (Layne, 1957 Kirk, 1947). These depend upon differences in the solubility, optical rotation, absorption below 230 m/ , or precipifability of unhydrolyzed protein and hydrolytic products. Such 

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