Mass spectrometry post-source decay analysis

B. Spengler. Post-Source Decay Analysis in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Biomolecules. J. Mass Spectrom., 32(1997) 1019-1036. 

Spengler, B. (1 997) Post-source decay analysis in matrix-assisted laser desorption/ionization mass spectrometry of biomolecules. Journal of Mass Spectrometry 32, 1019-1036. 

Berhane, B.T. and Limbach, P.A. (2003) Stable isotope labeling for matrix-assisted laser desorption/ionization mass spectrometry and post-source decay analysis of ribonucleic acids. 

Rehulka, P. Salplachta, J. Chmelik, J. Improvement of Quality of Peptide Mass Spectra in Matrix-Assisted Laser De-sorption/Ionization Time-of-Flight Mass Spectrometry and Post-Source Decay Analysis of Salty Protein Digests by Using on-Target Washing. J. Mass Spectrom. 2003,38,1267-1269. 

The development glycopeptide libraries obtained by the split-mix method is severely hampered by the lack of concurrent development of a general, facile separation and characterization technology. Some headway has been made with chemical coding of the libraries, but very few direct methods of analysis exist. One promising method that could be applied to the direct characterization of both types of libraries is mass spectrometry. More specifically, post-source-decay matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (PSD-MALDI-TOF-MS) and CID-FAB/MS/MS have been used to characterize glycopeptides.53-55... 

Several original papers must be mentioned that deal with mass spectrometric techniques which the numerous reviews do not comprise. Kaufmann and coworkers268,288 studied the mass spectrometric analysis of carotenoids and some of their fatty acid esters using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry and its post-source-decay (PSD) variant. Some advantages concerning the thermal instability and limited solubility were discussed, but the fragmentation paths of the carotenoid cations were found to be essentially the same as those observed with conventional techniques. 

Kaufmann, R., Wingerath, T., Kirsch, D., Stahl, W., and Sies, H. 1996. Analysis of carotenoids and carotenol fatty acid esters by matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source-decay mass spectrometry. Anal. Biochem. 238 117-128. 

B. Spengler, D. Kirsch, R. Kaufmann, and J. Lemoine, Structure analysis of branched oligosaccharides using post-source decay in matrix-assisted laser desorption ionization mass spectrometry, Org. Mass Spectrom., 29 (1994) 782-787. 

Lemoine, J., Chirat, F. and Domon, B. (1996) Structural analysis of derivatized oligosaccharides using post-source decay matrix-assisted laser desorption ionization mass spectrometry. J. Mass Spectrom., 31 (8), 908-12. 

In this study, we used matrix-assisted laser desorption ionization /Mass Spectrometry (MALDI/MS) to identify the peptides released from gastric parietal cell microsomes. MALDI, because of its sensitivity and relative tolerance to the presence of salts and buffers was examined for the analysis of unfractionated proteolytic digests (9, 10). MALDI with post-source decay (PSD) analysis was used to obtain sequence information on peptides even in crude digestion mixtures. Our strategy (Figure 1) consisted of proteolysis of intact vesicles, centrifugation at high speeds to separate membrane bound and soluble fractions and analysis of the mixture of released peptides by MALDI/MS. In addition, to increase the... 

S. Goletz, M. Leuck, P. Franke, U. Karsten. Structure analysis of acelylated and non-acetylated O-linked MUCl-glycopeptides by post-source decay matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectr. 1997,11, 1387-1398. 

T. Yamagaki, Y. Mitsuishi, H. Nakanishi. Post-source decay fragment analysis of a highly branched nonasaccharide of xyloglucan using M ALDI-TOF mass spectrometry. Chem. Lett. 1998,57-58. 

Al-Saad, K.A., Siems, W.F., Hill, H.H., Zabrouskov, V. and Knowles, N.R., Structural analysis of phosphatidylcholines by post-source decay matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry, J Am Soc Mass Spectrom, 14 (2003a) 373-382. 

Przybilla, L., Rader, H.-J, and Mullen, K., Post-source Decay Fragment Ion Analysis of Polycarbonates by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry, Eur. Mass Spectrom., 5, 133, 1999. 

Goldschmidt, R.J., Wetzel, S.J., Blair, W.R., and Guttman, C.M. (2000) Post-source decay in the analysis of polystyrene by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Am. Soc. Mass Spectrom., 11, 1095-1105. 

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