Mass Spectrometric Methods for Complete Structure

MASS SPECTROMETRIC METHODS FOR COMPLETE STRUCTURE ELUCIDATION OF OLIGOSACCHARIDES [Pg.401]

Currently, mass spectrometry has assumed a greater role in the characterization of oligosaccharides. This subject has been reviewed extensively [2-7]. A complete [Pg.401]

Structure elucidation of carbohydrate side chains requires knowledge of the (1) monosaccharide composition, (2) sequence and branching of each monomer, (3) stereochemistry, (4) interglycosidic linkage positions, and (5) anomeric configuration of each glycosidic bond. [Pg.402]

Prior to mass spectrometry analysis, the carbohydrate chain must be cleaved from glycopeptides and glycolipids. [Pg.402]

Glycolipid glycans and glycosaminoglycans are also released chemically. Digestion with endoglycoceramidase will also release glycolipid glycans. [Pg.402]

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