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Manganese Chloride Superoxide

A relatively recent indirect assay was set up by Paoletti (108). The assay consists of a sequence of reactions that generate superoxide from molecular oxygen in the presence of EDTA, manganese(II) chloride, and mercaptoethanol (108-110). The reactions are monitored by following the oxidation of NAD(P)H by superoxide radicals through a decrease of absorbance at 340 nm, which corresponds to the maximum absorbance of NAD(P)H. The addition of SOD (scavenging superoxide) inhibits nucleotide oxidation (Fig. 14). At variance with the previous assays, whereby C3itochrome c is reduced by superoxide, this method relies on the oxidation of NAD(P)H and, in theory, makes the detection less susceptible to aspecific reduction by common cellular components. The assay is very sensitive to the EDTA/Mn ratio and to the mercaptoethanol concentration, both of which affect nucleotide... [Pg.166]


See other pages where Manganese Chloride Superoxide is mentioned: [Pg.146]    [Pg.345]    [Pg.279]    [Pg.739]    [Pg.739]    [Pg.571]    [Pg.409]    [Pg.409]    [Pg.380]    [Pg.782]    [Pg.280]    [Pg.344]    [Pg.89]    [Pg.739]    [Pg.144]   
See also in sourсe #XX -- [ Pg.138 ]

See also in sourсe #XX -- [ Pg.138 ]




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Manganese chloride

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