Maleimide biotin-BMCC

Figure 11.8 Biotin-BMCC provides sulfhydryl reactivity through its terminal maleimide group. The reaction creates a stable thioether linkage.

The reagent is similar to another maleimide-containing biotinylation reagent, 3-(N-maleimi-dopropionyl) biocytin, a compound used to detect sulfhydryl-containing molecules on nitrocellulose blots after SDS-electrophoresis separation (Bayer et al., 1987). Biotin-BMCC should be useful in similar detection procedures. 

Fig. 8.5. Specific example of an RNA catalyzed reaction [4 + 2] Diels-Alder cycloaddition between an RNA-tethered diene and a biotin-linked maleimide dienophile (BMCC-biotin). Cycloaddition creates an affinity-tagged (biotin) RNA product that can be captured and purified.

---