MALDI AnchorChip

A Novel Approach Using MALDI-TOF/TOF Mass Spectrometry and Prestructured Sample Supports (AnchorChip Technology) for Proteomic Profiling and Protein Identification... 

Key Words MALDI TOF/TOF profiling biomarkers AnchorChip tandem MS magnetic beads clinical proteomics serum. 

This results in co-crystaUization of matrix and sample and therefore higher sample concentration focused at the distinct hydrophilic anchor points [16]. One of the most commonly used matrices is a saturated solution of a-cyano-4-hydroxy-cinnamic acid in 97% acetone/3% water containing a small amoimt of TFA to support the ionization of the sample molecules in the MALDI source. The matrix is directed to the AnchorChip target and this is then dried. The sample fractionation after HPLC can be performed automatically using special sample fractionation systems (e.g., Probot, LC Packings Dionex). The target is introduced to a special desk and can be adjusted in the three XYZ planes so that the small nano-LC volume is placed directly onto the matrix. Using this set-up, fractions of volume less than 40 nL can be collected automatically. After collection, the individual fractions are recrystallized from 0.01% TFA, 30% acetone, and 60% ethanol in water and analyzed. 

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