Magnetic gluing

BD, bipolar disorder GLU/GLN, glutamate/glutamine MRI, magnetic resonance imaging MRS, magnetic resonance spectros copy NC, normal control PFC, prefrontal cortex SCHZ, schizophrenia. 

The samples are contained in a demountable cell consisting of 2 CaF2 plates separated by a spacer of 10 or 20 micron. The sample cell is filled through two metal tubes drilled through and glued to the first CaF2 plate. The sample cell is held on to the Lissajous scanner by three magnetic supports. 

Selectively protected above diacid was converted to (y-N-Tfa-L-Glu-a-OMe)-L-Glu-L-Glu-tetra-OMe next way. To a solution of N-(y-N-Tfa-L-Glu-a-OMe)-L-Glu-L-Glu-a-OMe (0.18 g) in dry acetone (20 ml cooled to 0°C was added with magnetic stirring ethereal diazomethane (0.8 mole) until yellow color persisted. After evaporation of solvent, the colorless solid was recrystallized from methylene chloride-hexanes to afford 0.17 g (92%) of colorless crystals of (y-N-Tfa-L-Glu-a-OMe)-L-Glu-L-Glu-tetra-OMe MP 150°-152°C [a]D25= -8.5° (CHCI3). 

A 1.00 g (1.9 mmoles) of (y-N-Tfa-L-Glu-a-OMe)-L-Glu-L-Glu-tetra-OMe was dissolved under argon in dry dimethoxyethane (30 ml) with warming and magnetic stirring, and triethylamine (0.55 ml, 3.97 mmoles) was added. The reaction mixture was cooled to -30°C (dry ice isopropyl alcohol), and oxalyl chloride (0.35 ml, 3.97 mmoles) was added followed by dimethylformamide (2... 

Simulated nH NMR spectra of glutamine and glutamate at different magnetic field strengths. Linewidths (Av1/2) corresponded to values typical for very well shimmed volumes of the human brain. The concentration ratio [Glu]/[Gln] was set to 3 1. Frequency scale (Hz) is identical in all three spectra (From Tkac et al., 2001, with permission)... 

Fig. 3.—Nuclear Magnetic Resonance Spectra (Partial) at 100 MHz of (A) a-D-Glu-cose, and (B) a-D-Glucose-5,6,6 -d3 in Methyl Sulfoxide Solution, Showing Hydroxyl Proton Signals (OH) and the Anomeric Proton Signal (H-l). [The inset doublet in A represents the only signal (H-l) detected after the addition of deuterium oxide to the solution. ]...

Treatment of penta-0-acetyl-3-deoxy-3-C-(hydroxymethyl)-a-D-glu-copyranose (56) with hydrogen bromide in glacial acetic acid readily afforded the corresponding, branched-chain glycosyl bromide. Condensation of the latter with dithyminylmercury yielded a compound that is presumed (from its infrared and nuclear magnetic resonance spectra) to be the branched-chain nucleoside (62),... 

PJ.M. Folkers, G. M. Clore, P.C. Driscoll, Solution structure of recombinant hirudin and the Lys-47 glu mutant a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study. Biochemistry, 28 (1989) 2601. 

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