Magnesium , equilibrium binding studies

In spite of much information available for the interactions of various metal ions with small oxoanions of phosphorus, relatively little information has been obtained for the complex formation of long-chain polyphosphate ion. This may be due to the fact that the conventional methods useful for the study of the complex formation of a relatively small ligand are not always applicable to the polyanion complex formation system. Since a gel chromatographic method based on the same principle as the equilibrium dialysis method has been proved to be applicable in the field of inorganic complex chemistry (1), this method has been applied to the study of the binding of long-chain polyphosphate ions to magnesium ion. 

In order to study the effects of pH and pMg on the apparent equilibrium constant for the hydrolysis of ATP to ADP, the binding polynomials with magnesium terms for ADP and Pj are needed. 

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