ALDI-TOF MS

T. Yamagaki, Y. Mitsuishi, H. Nakanishi. Post-source decay fragment analysis of a highly branched nonasaccharide of xyloglucan using M ALDI-TOF mass spectrometry. Chem. Lett. 1998,57-58. 

Mass Spectrometry Facilities. The facilites of mass spectrometry (i.e., M ALDI-TOF, MALDI-TOF-MS/MS, LC-MS, LC-ESI-MS/MS) and other resources for data search and management. In most cases, each machine is designed for a particular purpose. 

In a recent work, improved calibration of M ALDI-TOF mass spectra was obtained by optimization of the electrostatic field generated by the ions. In contrast to conventional methods, where the relationship between ion flight time and mass is an arbitrary polynomial equation, this method is based on the physics of ion motion. Parameters needed to describe the physics are numerically optimized using a simple algorithm. Once these parameters are established, unknown masses can be determined from their times-of-flight. This calibration method gives well-behaved results, since non-linear parameters (due to extraction delay, desorption velocity, and other events) are properly taken into account in the time-of-flight calculation. ... 

M ALDI-TOF mass spectra of a dendrimer sample at various stages of its growth. The dendrimer grows on a star-shaped nucleus made of PEG. (Reprinted with permission from Ref. 298, Copyright 1999 American Chemical Society)... 

Santos, L.S., Haddad, R., Hoehr, N.R, Pilli, R.A., and Eberlin, M.N. 2004. Fast screening of low molecnlar weight componnds by thin-layer chromatography and on-spot M ALDI-TOF mass spectrometry. Ana/. Chem., 76 2144-2147. 

Teuber, K., Riemer, T., and Schiller, J. 2010. Thin-layer chromatography combined with M ALDI-TOF-MS and P-NMR to study possible selective bindings of phospholipids to silica gel. Anal. Bioanal. Chem., 398 2833—2842. 

Figure 17. Structural model of LAM and AG. AG is drawn without the mycolic acids attached to the terminal Ara residues. The molecular weight of LAM has been determined by M ALDI-TOF on both native and permethylated samples. The arabinan circle in both LAM and AG represents structural details not yet defined. Detailed characterizations of the various arabinan motifs were based on MS/chemical analysis of fragments obtained through acetolysis, mild acid hydrolysis and endoarabinase digestion as indicated. The branched Ara6 motif is common to both AG and LAM whereas the linear Ara4 terminal motif is found only on LAM. An Ara22 motif as drawn is deduced to be present on AG [15],...

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