Lysozyme distribution coefficient

Figure 5. Predicted and observed distribution coefficients for albumin and lysozyme.

Thomas, B. R., et al.. Distribution coefficients of protein impurities in ferritin and lysozyme crystals - Self-purification in microgravity. J. Cryst. Growth 2000,... 

Figure 7 Dependences of the size exclusion distribution coefficient, K, for the small globular protein hen egg white lysozyme versus the ionic strength, /, of the mobile phase for several notional size exclusion sorbents of different average pore diameter, particle size, and surface chemistry characteristics. The sorbents employed in these investigations were 1, Synchropak GPC 100 2, Waters I-125 3, Shodex OH Pak B-804 4, Lichrosorb Diol 5, Tosoh TSK SW 3000 and 6, Tosoh TSK SW 2000. The Interplay of hydrophobic interaction and electrostatic phenomena, superimposed upon the size exclusion effect due to the differences in the pore sizes of the support materials, is particularly evident with these sorbents at high- and low-ionic-strength conditions. (Data ad ed from Ref. 98.)...

Figure 11.4 Selective precipitation (i.e. phase split) for aqueous solutions containing two proteins, lysozyme and haemoglobin at pH 7 and ammonium sulfate - the salting out effect Ions of the electrolyte dehydrate the hydrophilic colloid, i.e. remove water from the polymer, whereby a phase split occurs where two liquids co-exist One of these phases is very dilute in protein while the other is highly concentrated and precipitation can take place. The experimental data show that when the ionic strength of ammonium sulfate exceeds about 7.5 M excellent separation of the proteins is obtained. K is the distribution coefficient of a given protein in the two liquid phases. Reprinted from Prausnitz (1995), with permission from Elsevier...

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