Lysozyme binding capacity

The ligand utilization of the metal chelate resin can be characterized by the ratio of ligand density to lysozyme binding capacity. This is shown in Fig. 10. 

Resin Epoxy group density [mmol g ] Ligand density IDA [mg mL [ Lysozyme binding capacity Cu -gel [mg mL [... 

Protein binding capacity per mL volume 6 mg lysozyme 3 mg lysozyme... 

A number of synthetic homologues of the loop-region (residues 64—82) of lysozyme that had amino-acid residues replaced singly or two at a time with L-alanine have been assessed in terms of their capacity to bind with anti-loop... 

Fig. 4.5. Structure of an hexasaccharide that can bind at the active center of lysozyme. Upon binding to the enzyme, the sugar ring D of the substrate becomes distorted and catalysis proceeds through the promotion of an oxocarbonium ion (see p. 228). This results in a polar transition state. However, an important feature of enzyme is the capacity to stabilize (neutralize) the enzyme-substrate complex by electrostatic interactions with amino acid residues at the active site.

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