Lysozyme antibodies bound

The objective of the experiments is to compare the affinity of wild-type antibody and wild-type immunogen (chicken lysozyme) with that of wild-type antibody and mutant antigen or with that of mutant antibody and wild-type immunogen. All the assays involve incubation of a constant concentration of one reactant with varying amounts of the complementary reactant, along with estimating the concentration of either bound or free reactant by an immunochemical or enzymatic method. In each case, the assumption is made that the measurement step does not disturb the equilibrium between antigen and antibody, and it is important that this assumption be validated experimentally. We summarize below several alternative methods. 

The DNP-DAS label bound between two large protein molecules by means of noncovalent binding to an anti-TNP antibody on one side and on the other side via covalent linkage to lysozyme using cyanuric chloride as the cross-linker [10]. (Reproduced with permission from Elsevier.)... 

An investigation by Raab et al. [197] also used AFM to aid recognition of antibodies, but this time using a magnetically oscillated cantilever, tethered to an antibody. This cantilever was scanned across a lysozyme-bound surface, where changes in the amplitude (in relation to the cantilever) high-... 

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