Lymphocytes microcytotoxicity tests

When leukocyte antigens were first detected in the 1950s, the assay consisted of a cumbersome agglutination technique. However, the introduction of the lymphocyte microcytotoxicity test by Terasaki and McClelland in 1964 (T6) eliminated many of the technical constraints inherent in the agglutination method and provided a more consistent and reproducible method for the demonstration of HLA products. The test has continued in use little changed since its introduction. 

Fortunately, hyperacute rejection may be largely eliminated by cross-matching the patient s serum against the donor lymphocytes using a variation of the lymphocyte microcytotoxicity test. The patient s serum is placed in the wells of a tissue typing tray and donor lymphocytes are added. The cells are examined for lysis after an appropriate incubation period and the addition of complement. If... 

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