Low O2-affinity

Boston a58(E7)His - Tyr. Permanently in deoxy form. Low O2 affinity. Low cooperativity. Bohr effect... 

Hemoglobin E P26(B8)Glu -> Lys. Purified HbE has normal O2 affinity. In erythrocytes homozygous HbE has low O2 affinity due to increased 2,3-diphosphoglycerate in ceils. Cells containing HbA and HbE (heterozygotes) have normal O2 affinity See section G. 

Helsinki p82(EF6)Lys -> Met. Low O2 affinity. Bohr effect decreased. Lysg2 is diphosphoglycerate binding site. 

Decreasing pH and increasing CO2 concentration lower hemoglobin s O2 affinity so that conditions of low pH and high carbon dioxide concentration promote... 

A general trend is evident that ease of Hb oxidation (lower 1/2) generally correlates with increased O2 affinity (low F1/2) for various Mbs, Hbs and their mutants. This is illustrated in Figure 2.9 where the Nernst plot for horse Hb is displaced towards lower potentials relative to that of human Hb Aq. One of the structural differences between human and horse Hb is the substitution of a... 

The rate constants for the association and dissociation of O2 and CO were determined by laser flash photolysis. The O2 affinity of the amide linked system was higher than that of the ether linked compound % 18.6 vs 2 torr) as a result of a difference of a factor of ca. 10 in the O2 dissociation rates (10 k 4 vs 0.5 s" ). This increase in stability of the amide oxygenated species was attributed to the presence of the N-H group and the possibility of hydrogen-bonding with the terminal oxygen atom. The low... 

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