Long-range NOE correlations

Figure 8. Key long range nOe correlations of patellamide C in CDCI3...

Eor a macromolecule such as a large protein, the steps in characterization involve, first, identification of the spin systems present, using correlated spectroscopy, and identification of neighboring amino acids. The long range noes are then assigned, and three bond coupling constants ate deterrnined. 

In order to determine the structure and stereochemistry of compounds 36 and 37, standard H- HCOSY, H- C shift correlation (HETCOR), DEPT, and H NOE NMR experiments were performed. The 13C NMR spectrum of compound 36 is based on DEPT, HETCOR, and long-range H- C shift correlation experiments, which allowed the correct assignment of most of the carbon signals <1997MI328>. 

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