Liquid immersion development equipment

A method has been developed that enables solid phase organic synthesis to be performed in microtiter wells not equipped with any kind of porous material at the bottom to facilitate the separation of solid resin beads from a solvent. The concept of washing resin beads in the Don Cucna synthesizer was developed by the need for a reliable and fast operational cycle applicable to a hundred reaction vessels at the same time. The simplest compact reaction block for solid phase synthesis is the 96-well plate. The suction (aspirating) principle of the Don Cucna synthesizer is based on the fact that in most solvents used in solid phase synthesis the resin beads settle to the bottom of the wells of the plates. The settling of the resin is relatively fast (tens of seconds). After the resin beads have settled, stainless-steel needles connected to an evacuated waste container are slowly immersed into the wells of a plate (Fig. 8). The needles remove the liquid from above the surface of the resin without disturbing the resin bed. For washing the resin beads in 96-well microtiter plates, two... 

Solid-phase microextraction (SPME) was developed in 1988 by Prof. Janusz Pawliszyn and his coworkers at Waterloo University in Ontario, Canada, and commercialized in 1993 by Supelco (28-32). Solid-phase microextraction is an innovative, completely solvent-free sample preparation method that can be used to concentrate flavors and fragrances and that significantly reduces the time and cost required for sample preparation it therefore represents an excellent alternative to conventional sample extraction techniques. The advantages of SPME are particularly remarkable because this solvent-free sample preparation method does not require any complex or expensive equipment. This highly effective technique can be used to concentrate volatile organic compounds from liquid samples (immersion SPME) or from the headspace above a liquid or solid sample (headspace SPME). 

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