Liposomes peptide haptens

Figure 22.16 SMPB-activated liposomes may be modified with peptide hapten molecules containing cysteine thiol groups. The resultant immunogen may be used for immunization purposes to generate an antibody...

Protocol for the Coupling of Peptide Haptens Containing Sulfhydryl Groups to Liposomal Vesicles... 

Dissolve a sulfhydryl-containing peptide hapten at a concentration of 25 pmol/ml in degassed, nitrogen-purged lOmM HEPES, 0.15M NaCl, pH 7.0. Add the peptide solution to the liposome suspension at a molar ratio necessary to obtain at least a 5 1 excess of thiol groups to the amount of maleimide groups present (as MPB-DPPE). 

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