Liposome cells

FIGURE 7 Various mechanisms of liposome-cell interaction, from Pagano and Weinstein, 1987.)... 

Allen, T. M., McAllister, L., Mausolf, S., and Gyorffy, E. (1981). Liposome-cell interactions A study of the interactions of liposome containing entrapped anti-cancer drugs with EMT6, S49 and AEl cell lines, Biochim. Biophys. Acta. 643. 346-362. 

Lee, K.-D., Nir, S. and Papahadjopoulos, D. (1993). Quantitative analysis of liposome-cell interactions in vitro rate constants of binding and endocytosis with suspension and adherent J774 cells and human monocytes, Biochemistry, 32, 889-899. 

Miller CR, Bondurant B, McLean SD, et al. Liposome-cell interactions in vitro effect of liposome surface charge on the binding and endocytosis of conventional and sterically stabilized liposomes. Biochemistry 1998 37 12875. 

Marty C, Meylan C, Schott H, et al. Enhanced heparan sulfate proteoglycan-mediated uptake of cell-penetrating peptide-modified liposomes. Cell Mol Life Sci 2004 61 (14) 1785-1794. 

In principle, liposomes can enter (target) cells through different pathways by direct fusion of liposomes and the plasma membrane (1) or by an endo-cytic uptake mechanism. Other liposome-cell interactions that have been described in the literature are absorption, phospholipid and protein exchange, and cell-induced leakage of liposome contents (2,3). 

Diizgiines N, Nir S. Mechanisms and kinetics of liposome-cell interactions. Adv Drug Deliv Rev 1999 40(l-2) 3-18. 

The correlation of monolayer properties and spherical membranes (liposomes, cells) has been discussed by Blume 44). 

Dimitriadis, G. J. Cellular uptake of ribonucleic acids entrapped into liposomes. Cell Biol. Int. Rep. 3 543-549, 1979. 

I 6 van den Bos EJ, Wagner A, Mahrholdt H, et al, Improved efficacy of stem cell labeling for magnetic resonance imaging studies by the use of cationic liposomes. Cell Transpl 2003 12 743-756. 

Weinstein J.N., S. Yoshikami, P. Henkart, R. Blumenthal, and W.A. Hagins (1977) Liposome-cell interaction transfer and intracellular release of a trapped fluorescent marker. Science 195, 489-492. 

Key words pH-sensitive liposomes, Cell penetrating peptide, TATp, Hydrazone, PEG-PE, Enhanced permeability and retention... 

Preparation of Liposomes for Leakage Assay and Liposome-Cell Interaction... 

Liposome-Cell Interactions with EJ Carcinoma Cells... 

Pagano and Weinstein [135] suggest that there are 4 possible types of liposome-cell interaction, namely stable adsorption, fusion, endocytosis and hpid transfer. These can all occur depending upon the experimental conditions and the hpid used for the construction of the hposomes. 

Figure 7.7-2. Liposome-cell interactions. Drug-loaded liposomes can specifically (A) or nonspecificaiiy (B) adsorb onto the ceil surface. Liposomes can also fuse with the cell membrane (C) and release their contents into the cell cytoplasm, or they can be destabilized by certain cell membrane components when adsorbed on the surface (D) so that the released drug can enter the cell via micro-pinocytosis. Liposome can undergo the direct or transfer-protein mediated exchange of lipid components with the cell membrane (E) or be taken up by specific or nonspecific endocytosis (F). In the case of endocytosis, a liposome can be delivered by the endosome into the lysosome (G), or en route to the lysosome, the liposome can provoke endosome destabilization (H), which results in drug liberation into the cytoplasm. (With permission from Ref. 29.)...

The measurements of the electrophoretic mobility of liposomes, cells, and organelles/ By this method, one can observe the -potential of the cell surface. It will be seen later (electrokinetic potential) that this potential ( ) can be related to the surface potential. 

Major Applications Chemical-mechanical polishing, stabilizing liposomes, cell motility... 

Key words Cell-free expression. Membrane proteins. Expression optimization. Detergents, Liposomes, Cell extracts... 

Matsuoka, K., Orci, L., Amherdt, M., Bednarek, S. Y., Hamamoto, S., Schekman, R., and Yeung, T. (1998). COPlI-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes. Cell 93, 263-275. 

Takei, K., Haucke, V., Slepnev, V., Farsad, K., Salazar, M., Chen, H., and De Camilli, P. (1998). Generation of coated intermediates of clathrin-mediated endocytosis on protein-free liposomes. Cell 94, 131-141. 

Allen TM, Sapra P, Moase E (2002) Use of the post-insertion method for formation of ligand-coupled liposomes. Cell Mol Biol Lett 7 889-894... 

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