Liposome affinity binding

Two optical sensor designs for biotin detection are described above. In this section, we present a liposome-amplified signalling strategy for electrochemical immunosensors, which converts the bio-specific affinity binding reactions into an electrochemical signal (Ho et al. 2009a). 

Q Yang, X-Y Liu, M Hara, P Lundahl, J Miyake. Quantitative affinity chromatographic studies of mitochondrial cytochrome c binding to bacterial photosynthetic reaction center, reconstituted in liposome membranes and immobilized by detergent dialysis and avidin-biotin binding. Anal Chem 280 94-102, 2000. 

Liu B. and Krieger M. (2002) Highly purified scavenger receptor class B, type I reconstituted into phosphatidylcholine/cholesterol liposomes mediates high affinity high density lipoprotein binding and selective lipid uptake. J. Biol. Chem. 277, 34125-34135. 

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