Lipofection procedure

For automation, the lipofection process was split of into four independent parts as follows (i) preparation of cationic liposomes, (ii) formation of lipo-plexes, (iii) transfection of the cells, and (iv) quantification of the lipofection efficiency and lipofection-induced cytotoxicity. As shown in Figure 1, this subdivision corresponds to the typical lipofection procedure and each part can be performed separately. 

Feigner PL, Gadek TR, Holm M, et al. Lipofection a highly efficient, lipid-mediated DNA transfection procedure. ProcNatl Acad Sci USA 1987 84 7413. 

Feigner, P.L., Gadek, T.R., Holm, M., Roman, R., Chan, H.W., Wenz, M. etal. (1987) Lipofection a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. USA, 84, 7413-7417. 

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