Lipid Extraction and TLC of Lipids

3 n-butanol/chloroform/glacial acetic acid/ddH20 60 10 20 10 (v/v/v/v) 

5 petrol ether/diethylether/glacial acetic acid 90 10 l(v/v/v) 

8 chloroform/methanol/acetone/glacial acetic acid/ddH20 75  

Freeze the tissue, which should be extracted, with liquid nitrogen, and pulverize it in a mortar under liquid nitrogen. 

Extract the tissue powder with 1 ml/g of Soln. A in a glass/Teflon 

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