Lipases Production, Isolation, and Purification

Fermentation is usually followed by the removal of cells from the culture broth, either by centrifugation or by filtration (Westoby et al., 2011). The cell-free broth is then concentrated by ultrafiltration, ammonium sulfate precipitation, or extraction with an organic solvent (Saxena et al., 2003). A single purification step is usually not adequate to get lipase of high purity. Therefore, purification using a combination of chromatographic techniques is mainly employed. However, except in the pharmaceutical industry, pure lipases are required and crude enzymes are usually adequate. 

Supercritical Fluids Technology in Lipase Catalyzed Processes 

---