Lipase Achromobacter

Hydrolysis of Polyester by Lipase. Aliphatic polyester, PEA and PCL were hydrolyzed by lipases from Achromobacter sp., C. cylindracea,... 

Microorganisms found in the microflora from holding tanks belong primarily to the genera Pseudomonas, Alcaligenes, Enterobacter, and Achromobacter. However, Pseudomonas predominates, and isolates from bulk milk show much more lipolytic and proteolytic activity than other psychrotrophs isolated (Stewart et al. 1975). Bacterial exocellular lipases have an optimum pH of 8.75, a relative optimum temperature at 37°C, and an absolute optimum temperature at 50°C (Driessen and Stadhouders 1974B). Kishonti (1975) reported two optimum temperatures at 30° and 55°C, respectively. 

Lipase preparations from numerous microorganisms, including those mentioned above, have been used in the synthesis of dairy (buttery or cheesy) flavors from milk fat (Arnold et al., 1975 Kilara, 1985) or to enhance flavor development in ripening cheese (Fox, 1988). These include lipases from Rhizomucor (Mucor) miehei (Moskowitz et al., 1977 Huge-Jensen et al., 1987), Achromobacter lipolyticum (Khan et al., 1967), Aspergillus niger... 

Khan, I.M., Dill, C.W., Chandan, R.C., Shahani, K.M. 1967. Production and properties of the extracellular lipase of Achromobacter lipolyticum. Biochim. Biophys. Acta 132, 68-77. 

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