Lineweaver-Burk and Eadie Analyses

Several methods have been developed for analyzing rate data for enzyme-catalyzed reactions. One of the most commonly used methods is based on writing Eq. (6.14) in the reciprocal form 

Therefore, a plot of [S]/R versus [S] will be Hnear with a slope of 1 /k2 [E]j, which is equal to and an intercept ofKm/fe2[E]t, which is Km/i max  

FIGURE 6.5 A Lineweaver-Burk plot for an enzyme-catalyzed reaction. 

Performing the multiphcation on the left-hand side of this equation gives 

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