Linear chamber

Horizontal, one-dimensional development in a linear chamber (Camag). 

When TLC is compared with other separation techniques, it has many disadvantages but also some advantages, the most important being simultaneous development of standards and samples on the same plate. It is possible to spot 74 samples on both sides of a plate and develop it in a linear chamber. It is interesting that this unique option has had so small an influence on modem developments in quantitative TLC. 

For the separation of aminoglycoside antibiotics with a linear chamber on silica gel HPTLC plates (Merck), the solvent methanol/25% ammonia (2 3) was used (31). The dipping reagent was 0.3 g of ninhydrin + 100 ml of 1-butanol + 3 ml of acetic acid. The / y values were... 

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