Light scatter side

Fig. 1. Orthogonal light scatter (side scatter, SSC) (y-axis) displayed against forward scatter (FSC) (x-axis) for the author s peripheral blood leucocytes. This is in the form of a dot plot. Each dot represents one cell. A gate has been set on the lymphocyte cluster. If there is any doubt, this cluster can be positively identified using a pan-T antibody (anti-CD3) and back-gating, as described in Section 3.2.2. Data were recorded using a Becton-Dickinson FACSCAN.

Figure 5B. Correlation of right-angle light scatter measured by fluorometry and flow cytometry. The top panel shows flow-cytometric data of side scatter of fixed, stained cells during the time course of stimulation by 1-nM (solid line, solid circles) or 0.01-nH (dashed line, open circle) FLPEP. The bottom panel shows the corresponding right-angle light-scatter data acquired pseudo-simultaneously on live cells in the fluorometer. The flow-cytometric data have been averaged, but the fluorometry data are plotted for both duplicates from one donor. Reproduced with permission from Ref. 27. Copyright 1985 Rockefeller University Press.

Fig. 1.5.6 Polarization ratio of the scattered lighi against the angle for the unreacted titanium (IV) ethoxide, Ti(OEt)4, aerosol generated at 101°C, and the same aerosol reacted with water vapor at three distances (X) corresponding to 13, 39, and 340 ms reaction time. On the right-hand side are given calculated modal radii and refractive indices from the light scattering data. The bottom values are for the solid titania. (From Ref. 37.)...

FSC]), strongly related to cell size) against light scattered at a wide angle (side scatter [SSC], which is influenced by cell structure and granularity). 

---