Light emission, from chemically generated fluorescent molecule

To our surprise and satisfaction, the general approach worked the CBI derivatives did chemiluminescence, and the sensitivity enhancement was 30- to 50-fold over fluorescence With this success, we embarked on a more thorough study of chemiluminescence with the goal of optimizing the method. Identifiable parameters that affected the efficiency of light emission from a chemically generated fluorescent molecule included ... 

Abstract Photochemistry is concerned with the interaction between light and matter. The present chapter outlines the basic concepts of photochemistry in order to provide a foundation for the various aspects of environmental photochemistry explored later in the book. Electronically excited states are produced by the absorption of radiation in the visible and ultraviolet regions of the spectrum. The excited states that can be produced depend on the electronic structure of the absorbing species. Excited molecules can suffer a variety of fates together, these fates make up the various aspects of photochemistry. They include dissociation, ionization and isomerization emission of luminescent radiation as fluorescence or phosphorescence and transfer of energy by intramolecular processes to generate electronic states different from those first excited, or by intermo-lecular processes to produce electronically excited states of molecules chemically different from those in which the absorption first occurred. Each of these processes is described in the chapter, and the ideas of quantum yields and photonic efficiencies are introduced to provide a quantitative expression of their relative contributions. 

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