Ligand binding reaction pseudo first order

Kinetic traces acquired under pseudo-first-order conditions can be fitted to exponential functions, and the observed rate constants, kobs, can be calculated. The second-order rate constants can be obtained from the slopes of the linear plots of kobs versus [hgand] (e.g. the ligand-binding reactions). The activation parameters can be determined through a systematic variation of temperature and pressure. The activation enthalpies and entropies, AH and A5, are calculated using the Eyring equation (1), and the volumes of activation, AV, calculated from the slope of In kobs versus pressure (under certain conditions). 

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