Ligand binding assay molecularly imprinted polymers

Application of molecularly imprinted polymers in competitive ligand binding assays for analysis of biological samples... 

Optimization of molecularly imprinted polymers for radio-ligand binding assays... 

Mayes, A. Lowe, C.R. Optimization of molecularly imprinted polymers for radio-ligand binding assays. Royal Society of Chemistry Cambridge, UK, 1998 Vol. 25, 28-36. Andersson, L.I. Muller, R. Vlatakis, G. Mosbach, K. Mimics of the binding sites of opioid receptors obtained by molecular imprinting of enkephalin and morphine. Proc. Natl. Acad. Sci. USA 1995, 92, 4788 792. 

Andersson, L.I. Application of molecularly imprinted polymers in competitive ligand binding assays for analysis of biological samples. In Molecularly Imprinted Polymers Man-made Mimics of Antibodies and Their Applications in Analytical Chemistry, Sellergren, B., Ed. Elsevier Amsterdam, 2001 341-354. 

Molecularly imprinted sorbent assays represent one of the most typical applications of biomimetic use, where imprinted polymers are used as substitutes of natural antibodies in immunoassays. The assays usually involve competitive binding of an analyte with a certain quantity of labeled ligands, in which the labeled ligand unbound is proportional to the analyte added. Because dissociation constants of common imprinted polymers are around 10 6-10 9 M, competitive binding assays could easily be performed. In practice, many molecularly imprinted sorbent assays have been developed for biologically active compounds, including theophylline, diazepam [26], S-propranolol [27], morphine, Leu-enkephalin [28], cyclosporin A [29], yohimbine [30], methyl-a-glucoside [31], corticosteroid [32], atrazine [33, 34], and 2,4-D [35]. 

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