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Liebermann-Bouchard reagent

There are different types of tests for detecting the presence of triterpenes in an extract. The Liebermann-Bouchard reagent is the one most frequent used to differentiate triterpenes and steroidal aglycones, whereas Zimmermann s test is appropriate for 3-oxo triterpenoids. [Pg.108]

Fig. 1.- Thin layer chromatography of esterified cholesterol. Cholesterol esters were purified according to Horning et al. (I960) and run on TLC with heptane-ethyl ether (24 1). Spots were visualized with Liebermann-Bouchard reagent. Cholesterol oleate standard (St). Material from animals injected with Freund s adjuvant (FC) white matter (WM)j myelin (My) apoprotein of Folch s proteolipid (AFP) basic protein (BP) basic protein plus CNS lipids (BPL). Fig. 1.- Thin layer chromatography of esterified cholesterol. Cholesterol esters were purified according to Horning et al. (I960) and run on TLC with heptane-ethyl ether (24 1). Spots were visualized with Liebermann-Bouchard reagent. Cholesterol oleate standard (St). Material from animals injected with Freund s adjuvant (FC) white matter (WM)j myelin (My) apoprotein of Folch s proteolipid (AFP) basic protein (BP) basic protein plus CNS lipids (BPL).
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