Leucine formation, free energy

In plastein synthesis the free energy of formation of the peptide bonds is small, as attested to by the reversal of hydrolysis merely by concentrating certain enzymatic hydrolytic products the synthetic product is insoluble, which tends to drive the reaction toivards synthesis. In these two respects plastein formation resembles reactions of the type benzoyl-L-leucine - - L-leucine anilide to benzoyl-L-leucyUeucine anilide. 

The free energy of formation of the peptide bonds in plastein synthesis must be small this is attested to by the possibility of inducing peptide bond synthesis merely by concentrating an enzymatic hydrolyzate. The synthetic product is practically insoluble and this drives the reaction toward synthesis. It is in these two respiects that plastein formation resembles reactions of the type benzoyl-L-leucine + L-leucine anilide —> benzoyl-L-leucylleucine anilide. 

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