Learn Identification of Metabolic Burdens During Production

2 Learn Identification of Metabolic Burdens During Production 

One of the earliest applications of omics analysis to ethanol production was the use of DNA arrays to compare transcript abundance in E. coli KOll and its evolved derivative LYOl, which has improved ethanol tolerance and production relative to KOll [146]. This comparison showed that several pathways involved in the production of osmoprotectants, such as glycine and betaine, had increased expression in the evolved strain. Provision of these osmoprotectants to the parent strain improved growth in the presence of ethanol [146] and ethanol production [147]. This finding inspired later engineering efforts to improve organic solvent tolerance in E. coli [148]. 

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