LC-NMR optimisation

For many practitioners of LC-NMR in pharmaceutical development, the LC method is pre-determined by that developed by the analytical chemist. 

Broadening of peaks in the flow system present yet another problem for sensitivity and can mean that even less of the analyte peak is used, even if it would appear narrow enough to be totally contained in the flow cell. Broadening of peaks during Ailing of the cell is much larger for peaks that are narrower than the volume of the cell compared with those that are larger than the cell volume [58]. This can lead to a further deterioration of LC-NMR sensitivity by a factor of 2 for narrow peaks [57]. 

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