Largomycin isolation process

Process Developments in the Isolation of Largomycin F-II, a Chromoprotein Antitumor Antibiotic... 

Prior to attempting any process development work, it was decided to evaluate the process reported by Yamaguchi et at (2) for the isolation of largomycin F-II. 

NCI-FCRF Process II-B (Scheme 5). In this process the freeze-thaw step of mycelia suspension, to liberate largomycin F-II, of NCI-FCRF Process II-A has been replaced by vigorously mixing the mycelia suspension for 1 hour. The results of a 240 gallon fermentation run are summarized in Scheme 5. A comparison of the properties of the isolated product with the standard compound is shown in Table II, and their polyacrylamide gel electrophoresis pattern is shown in Figure 5. 

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