Lactone ring-opening polymerization CALB

If we apply the generally assumed mechanism of the CALB-catalyzed ringopening polymerization of lactones [7h] to the polymerization of P-lactam the first step would be formation of a first acyl-enzyme intermediate as a consequence of the ring-opening of P-lactam by Ser10s assisted by His 224 and Aspi87 as depicted in Figure 14.8. 

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