Freeze-drying Lactic Acid Bacteria

Jabrait, A. 1969. Influence of coagulation and freeze-drying on survival of lactic acid bacteria in bioghurt. Lait 49, 520-532. (French)... 

Ishibashi, N., Tatematsu, T., Shimura, S., Tomita, M., and Okonogi, S., Effect of water activity on the viabiltiy of freeze-dried bifidobacteria and lactic acid bacteria, in I.I.E.-I.I.R-Commission Cl, Tokyo, Japan — 1985/1,227-32 (1985). 

To, B.C.S. and Etzel, M.R. Spray drying, freeze drying or freezing of three different lactic acid bacteria species, /. Food Scl, 62, 576,1997. 

C.F. De Valdez, G.S. De Giori, and A.P. De Ruiz Holgado, Effect of drying medium on residual moisture content and viability of freeze-dried lactic acid bacteria. Applied and Environmental Microbiology, 49,413 15 (1985). 

Microorganisms are commonly stored on the appropriate solidified medium, either as slants or stabs (weeks) or for longer periods (months) as liquid cultures suspended in glycerol kept at low temperatures (—70°C/ —94°F). Alternatively, microorganisms can be preserved for very long term storage (years) by freeze-drying. Method 13.10.3 has been used to freeze-dry yeasts (C.M.L. Joseph, personal communication, 2005), and method 13.10.4 has been used successfully for lactic acid bacteria (Duke, 1979). 

FIGURE 39.17 Effect of the protective substance s medium on the survival rate of Lactobacilli at various dr dng times (1) adonitol (2) glutamate (3) PEG 1000 and (4) nonfat skim milk. (From C.F. De Valdez, G.S. De Giori, and A.P. De Ruiz Holgado, Effect of Dr dng Medium on Residual Moisture Content and Viability of Freeze-Dried Lactic Acid Bacteria, Applied and Environmental Microbiology, 49, 413-415 (1985). With permission.)... 

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