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Labeling with sulfonyl hydrazine

Texas Red hydrazide is a derivative of Texas Red sulfonyl chloride made by reaction with hydrazine (Invitrogen). The result is a sulfonyl hydrazine group on the No. 5 carbon position of the lower-ring structure of sulforhodamine 101. The intense Texas Red fluorophore has a QY that is inherently higher than either the tetramethylrhodamine or Lissamine rhodamine B derivatives of the basic rhodamine molecule. Texas Red s luminescence is shifted maximally into the red region of the spectrum, and its emission peak only minimally overlaps with that of fluorescein. This makes derivatives of this fluorescent probe among the best choices of labels for use in double-staining techniques. [Pg.429]

Lissamine rhodamine B sulfonyl hydrazine is a hydrazide derivative of sulforhodamine B that can spontaneously react with aldehyde- or ketone-containing molecules to form a covalent, hydrazone linkage (Fig. 217). It also can be used to label cytosine residues in DNA or RNA by use of the bisulfite activation procedure (Chapter 17, Section 2.1). The resulting fluorescent derivative exhibits an excitation maximum at a wavelength of 556 nm and a maximal emission wavelength of 580 nm when dissolved in methanol. In... [Pg.348]

The hydrazide derivative can be used to modify aldehyde- or ketone-containing molecules, including cytosine residues using the bisulfite activation procedure described in Chapter 17, Section 2.1. The sulfonyl hydrazine group of Texas Red hydrazide reacts with aldehydes or ketones in target functional groups to form hy-drazone bonds (Fig. 218). Carbohydrates and glycoconjugates can be specifically labeled at the polysaccharide portion if the required aldehydes are first formed by periodate oxidation or another such method (Chapter 1, Section 4.4). [Pg.350]

Finally, another group of derivatives are the aldehyde-/ketone-reactive probes. This group is based on the activation of a sulfonyl hydrazine group of carbon number 5. They are based on Lissamine and Texas Red structures and used to label aldehyde-/ketone-containing molecules (with sugars). The most common aldehyde-/ ketone-reactive probes are Lissamine rhodamine B hydrazine and Texas Red hydrazine. [Pg.1232]


See other pages where Labeling with sulfonyl hydrazine is mentioned: [Pg.427]    [Pg.429]   
See also in sourсe #XX -- [ Pg.428 ]




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Labeling with

Labelled with

Sulfonyl hydrazine

With hydrazine

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