Labeling target specific

Figure 10 Mismatch detection by using a chemiluminescent AE-labeled cDNA probe. Procedure [9, 11] Acridinium ester-labeled probes specific for either wild-type or mutant sequence corresponding to a target DNA are hybridized with the sample DNA for 1.0 h at 60°C in a hybridization buffer (pH 5.2). Hybridized and nonhybridized probes are discriminated by the hydrolysis reaction for 12 min at 62.5°C in the presence of Na2B407 (pH 8.5) and Triton X-100. The chemiluminescence of each sample is then measured in a luminometer.

Add enzyme-labelled antibody, specific for the target antigen, to the solid phase. The "tagged" antibody will attach to any antigen captured by the solid phase antibody. 

Despite many difficulties, efficient targeted MRI contrast agents have been developed based on antibodies. Low concentration of receptor sites has been tackled with and elegant solutions have been proposed. Antibody labeling may therefore find a place in the arsenal of target specific MRI contrast agents. 

FIGURE 9.16 Principle and procedure of the sequence-specific DNA detection in the microfluidic system under laminar flow conditions. The labeled DNA was indicated by a circular dot. Fluorescence of the duplex was measured at B, and that of labeled target was measured at A [1177]. Reprinted with permission from the Royal Society of Chemistry. 

---