Labeling controls hydrogen peroxide

Figures. Comet assay is shown for human K562 cells exposed to an extract produced from particulate matter released from sample PM 5 filters, to an extract derived from an unloaded filter, or to hydrogen peroxide (100 pM) as a control. Cells with little or no DNA damage are labeled 1 and 2, and those with extensive damage are labeled 3 and 4.

An attempt to distinguish the two by lsO labeling was frustrated by exchange in the perrhenate product. However, the authors favored the latter mechanism, based on the observation that the observed rate constants suggested that kA or /cMto had to be near the diffusion-controlled limit, coupled with the known higher nucleophilicity of the hydroperoxide vs hydroxide anions.37 The formation of the new C—O bond in this case is thus reminiscent of the oxidation of alkylboranes with hydrogen peroxide.38... 

Fig. 1.13 Calibration of 8-sensor microfluidic array with off-line analyte capture by multilabel HRP-MP-Ab2 particles using 200,000 HRP labels/particle for PSA and IL-6 mixtures in serum. Signals developed by injecting 1 mM hydroquinone mediator + 100 pM hydrogen peroxide as enzyme activator, c Simultaneous determinations by the array compared to individual ELIS As for PSA and IL-6 in patient serum 1-4 are from prostate cancer patients 5 is a cancer-free control. Reproduced with permission from ref. 112 copyright Elsevier, 2011...

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