2-Keto-3-deoxy-6-phosphogluconic acid

In a certain group of bacteria, still another pathway (Entner-Doudomff pathway) for the utilization of glucose has been studied. Here glucosc-6-phosphate is oxidized to 6-phosphogluconic acid which is dehydrated to 2-keto-3-deoxy-6-phusphogluconic acid. This substance is then split to pyruvic acid and glyceraldehyde-3-phosphatc l which alsu can be converted to pyruvic acid). 

The oxidative decarboxylation of 6-phosphogluconic acid occurs in a stepwise mechanism involving a )8-keto acid intermediate 64). However, it appears that no metal ion is required in the reaction (66). 2-Deoxy-6-phosphogluconate is also a substrate for this enzyme, but the reaction is about two orders of magnitude slower than reaction of the natural substrate. The keto acid produced in the initial oxidation is released into solution and subsequently undergoes slow enzyme-catalyzed decarboxylation (67). 

Keto-3-deoxy-6>phosphogluconic add was first isolated as a product of the action of a bacterial enzyme (Pseudomonas sauharophyla) on 6-phos-phogluconic acid . It is also formed from ATP and 2-keto-3-deoxy-D-gluconic add with an enzyme from E. coli. ... 

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