KcsA PDB

Figure 1 Examples of several bacterial membrane proteins. The outer membrane (OM) of Gram-negative bacteria contains exclusively fS-barrel proteins, and three examples are shown BtuB (PDB ID 1NQF), which is the 22 p-stranded TonB-dependent active transporter for vitamin B 2/ th LamB or maltoporin trimer (PDB ID 1AF6), which is the 18 p-stranded passive sugar transporter and OmpA (PDB ID 1BXW), which is an 8 p-stranded protein that provides structural support for the OM. Proteins in the cytoplasmic membrane (CM) are helical, and three examples are shown the potassium channel KcsA (PDB ID 1BL8), which is a tetramer Sec YEG (PDB ID 1RH5), which forms the protein transport channel in Methanococcus and BtuCD (PDB ID ...

The simulated 2D PISEMA spectra and crystal structures of KcsA (PDB... 

In Figure 5 of reference 16, the pore region is based on the KcsA K channel from PDB 1BL8. Co-crystallized with Fab monoclonal antibodies from mouse. 

In cases such as KcsA, where there are many PDB entries for the same general structure, an example PDB ID is given. The Turret is not a domain per se, rather an extracellular loop between S5 and the pore helix of the pore domain. 

PISEMA spectra of ion channel proteins (A) crystal structure and (B) simulated PISEMA spectrum of a 10.2 kDa KcsA monomer (97 residues), a K+ channel of S. lividans (PDB ID 1BL8) (C) crystal structure and (ID) simulated PISEMA spectrum of a monomeric 50.3 kDa (473 residues) CLC chloride channel from E. coli (PDB ID IKPK). 

Fig. 5 Inactivation of K channel, a) Crystal structure of KcsA channel with a hydrophobic cation, tetrabutylammonium TEA. b) Composite model of a voltage-dependent channel. The a-subunit is shown in blue and the P-subunit in red. The pore is represented by the KcsA K channel (5) and the Tl-P complex (15). The structures of the linker (Tl-Sl) connecting the voltage sensors to the TI domain are unknown. An N-terminal inactivation gate is shown entering a lateral opening to gain access to the pore. Fig. 5a was produced with a PDB file (lJ95.pdb from Ref. [14]) and Raswin Molecular Graphics (version 2.7.2. Glaxo Research and Development. U.K.).

Figure 2 Two sub-units of the KcsA (left) and MthK (right) potassium channels embedded in an explicit POPC lipid bilayer. The atoms lining the selectivity filter are represented as spheres to show the individual cages which represent the binding sites of K+ ions. The KcsA and MthK structures are obtained from the protein database codes IblS.pdb" and llnq.pdb," respectively.

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