Kalihinol metabolites

To separate homogeneous compounds from co-eluting metabolites, repeated HPLC experiments with changes in column and solvent systems were necessary. For example, kalihinol-A (107) sharing similar retention times with kalihinol-C (114), and kalihinol-F (112) with kalihinol-E (108), were resolved successfully on an ODS reverse phase column. Crystallization experiments were repeatedly undertaken. The sample of kalihinol-F (112) prepared for X-ray analysis had two C22H33N3O2 molecules in its asymmetric unit. 

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