JS-D-Mannanase

A way of reconciling the observed proportions of mannobi-, tri-, and tetra-oses and heterosaccharides,16113 released by guar-seed jS-D-mannanase (from hot-water-soluble carob galactomannan), with this model, would be if the enzyme preferentially hydrolyzes appropriate, unsubstituted d-mannosyl residues (as in 31) then, it would only be able to hydrolyze on the reducing side of a substituted D-mannose (as in 32) if there were a... 

A simple assay procedure for j5-D-mannanase enzyme which has been developed employs carob D-galacto-D-mannan dyed with Remazolbrilliant Blue." Additionally, the procedure is quantitative, relatively sensitive, and highly specific for jS-D-mannanase enzyme. It can be readily used for the determination of jS-D-mannanase activity in crude enzyme preparations and column-chromatography eluates. 

Germinating lettuce Lactuca sativa) seeds synthesize a jS-D-mannanase that hydrolyses the o-mannan in the endosperm wall. ... 

The jS-D-mannanase activities in germinating seeds of lucerne, guar, carob, and soybean could be separated from the accompanying a-D-galactosidase activities only with difficulty. The role of the jS-D-mannanases in degrading the galactomannan in cotyledon embryos was discussed. 

An enzyme preparation from guar,236 described as exo-jS-D-(l— 4)-mannanase, hydrolyzed ivory-nut mannan almost completely to D-mannose. The purified (1,000-fold) enzyme from pineapple300 hydrolyzed the substrates in the order p-nitrophenyl /3-D-mannopy-ranoside >/3-D-Man-(GlcNAc)2-Asn (a core glycopeptide) > methyl /3-D-mannopyranos ide > /3-D-Man-(l—>4)-d-G1c > /3-D-Man-(l—>4)-D-mannitol. 

The modes of action of jS-o-mannanases of diverse origin on legume seed galactomannans have been reported. The enzymes have different abilities to hydrolyse galactomannans especially when the backbone is highly substituted with D-galactose residues. 

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