Isotype Determination by EIA AP Conjugate

This protocol describes the use of an alkaline phosphatase conjugate of course, a HRP conjugate works well, too. 

Dilute the class-specific capture antibody to 2 pg/ml in Soln. A. Apply 100 pl/well of the coating solution to a microtiter plate and incubate at 4 °C overnight. Remove the liquid from the plate and wash once with Soln. B. Block the wells with 150 pi Soln. C at RT for 2 h. Knock out the blocking solution and wash once with Soln. B. Store the plate in a sealed bag at 4 °C. 

---